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So, why is this exciting and how does it work? Well firstly, electron microscopy is cool. Instead of a person trying to pick out features, the features are standing out for themselves in brilliant color. The importance of this new technique is that it is the sample itself speaking. The Emergence of Color Transmission Electron MicroscopyĪ research group headed by Ellisman and Tsian at the University of California, San Diego, has recently developed an exciting new technique, whereby TEM samples themselves can express color. For a more comprehensive introduction on to electron microscopy, check out one of our previous articles on the topic. This makes it easy to miss tiny details and differences between samples.
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While these efforts can improve clarity in the image, the final result is a product of human interpretation.
#DISADVANTAGES OF ELECTRON MICROSCOPE SOFTWARE#
To produce contrast and to highlight particular features, TEM images are often false colored using software such as PhotoShop or ImageJ. Variation in the signal across the detector produces the features in the image (i.e., where the signal is strong, the image is bright where the signal is weak, the image is dark). This is because the detectors used in a TEM detect charge (as opposed to detecting light like in a regular microscope), so the signal that forms the image is binary and the image is produced in greyscale. However, one of the limitations of TEM images is that they are black and white. TEM gives us an excellent view into the ultrastructure of tiny features inside the cell. How Does TEM Work? – Here Comes the Science Bit! TEM works by shooting electrons through the sample, and its resolution is so fine it can image individual ribosomes. TEM is a super-resolution imaging technique that produces a 2D image using electrons instead of photons. While SEM images are aesthetically amazing, the TEM images bring us inside the world of the cell. I highly recommend searching for SEM samples on Google images. SEM creates fascinating 2D images by bouncing electrons off the surface of the sample. There are two types of electron microscopy-transmission electron microscopy (TEM) and scanning electron microscopy (SEM).